A universal method of purifying genetically engineered human somatotropin and its mutants using metallochelating agent affinity chromatography
Lopatin, S.A.; Il'ina, A.V.; Shul'ga, A.A.; Grinchenko, O.S.; Varlamov, V.P.; Skriabin, K.G.; Kirpichnikov, M.P.
Biokhimiia 59(2): 226-230
1994
ISSN/ISBN: 0320-9725 PMID: 8155782 Document Number: 427606
A novel scheme for human recombinant somatotropin and its mutant forms isolation which includes ion-exchange and metal chelate affinity chromatography has been developed. A change in the chromatographic behaviour of the isolated proteins depending on the amino acid substitutions has been demonstrated.
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