An effective method of purifying recombinant human tumor necrosis factor alpha
Tikhonov, R.V.; Iakimov, S.A.; Korobko, V.G.; Vul'fson, A.N.
Bioorganicheskaia Khimiia 22(3): 163-167
1996
ISSN/ISBN: 0132-3423 PMID: 8687507 Document Number: 466168
An efficient and productive isolation method for human recombinant tumor necrosis factor alpha from Escherichia coli cells was developed. The method includes a membrane filtration step, two steps of ion-exchange chromatography, and gel filtration on a Sephadex G-25 column. The target product was obtained with approximately 50% total yield and greater than 95% purity according to PAGE and HPLC.
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