Biosynthesis and isolation of a recombinant protein for producing genetically-engineered human proinsulin
Ivankin, A.N.; Mitaleva, S.I.; Nekliudov, A.D.
Prikladnaia Biokhimiia i Mikrobiologiia 34(3): 293-299
1998
ISSN/ISBN: 0555-1099 PMID: 9644711 Document Number: 490246
Isolation of the recombinant protein from a genetically engineered Escherichia coli 1854 producer for further chemical enzymatic transformation into human insulin through proinsulin was studied. Under optimal conditions, the recombinant protein formation was more than 35% of the total cell proteins. Structures of the polypeptides obtained and purified chromatographically were confirmed by amino acid analysis. Human proinsulin was derived from the recombinant protein isolated.