Use of monoclonal antibodies for purifying the regulatory subunit of cAMP-dependent protein kinase
Eshba, I.R.; Kochanova, N.A.; Popov, K.M.; Bulargina, T.V.; Severin, E.S.
Biulleten' Eksperimental'noi Biologii i Meditsiny 103(5): 567-569
1987
ISSN/ISBN: 0365-9615 PMID: 3297186 Document Number: 303629
Monoclonal antibodies against regulatory subunit of cAMP-dependent protein kinase, type II, were obtained from pig brain (R II). The immune-affinity sorbent has been synthesized on the basis of monoclonal antibodies against R II. The method was proposed for the purification of homogeneous R II with high cAMP-binding activity using immune-affinity sorbent.
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