Detection of antibodies to Aspergillus fumigatus in serum of horses with mycosis of the auditory tube diverticulum (guttural pouch)
Guillot, J.; Sarfati, J.; Ribot, X.; Jensen, H.E.; Latgé, J.P.
American Journal of Veterinary Research 58(12): 1364-1366
1997
ISSN/ISBN: 0002-9645 PMID: 9401681 Document Number: 478607
Blood samples were collected from 12 clinically healthy horses and 12 horses with guttural pouch mycosis (GPM), diagnosed by endoscopic observation of characteristic mycotic plaques. Antibodies to A. fumigatus antigens were detected in serum by use of an ELISA and immunoblot analysis with extracellular antigens. Antibodies against A. fumigatus antigens were found in both healthy and diseased horses. Titre of total Aspergillus antibodies was not diagnostic for GPM. In contrast, immunoblot analysis results indicated that 2 antigens of 22 and 26 kDa were constantly recognized by sera from diseased horses.