Protection of ebselen on oxygen free radical-induced lipid peroxidation damage of cultured rat cortical neuron and cortical mitochondria
Tan, Y.X.; Li, W.H.; Jiang, Y.Y.; Tao, X.B.; Chen, W.P.
Yao Xue Xue Bao 32(3): 166-170
1997
ISSN/ISBN: 0513-4870 PMID: 11327014 Document Number: 475146
Ebselen (2-phenyl-1, 2-benzisoselenanzol-3 (2H) one, C-13 H-9NOSe) is a selenoorganic anti-oxidant compound. In this study, the effect of ebselen on lipid peroxidation damage induced by O-2- and cntdot OH in vitro, of cultured rat cortical neuron and cortical mitochondria was studied. When neuron was exposed to hypoxanthine/xathine oxidase system and vitamin C/CuSO-4 system, obvious damage was dectected: lactic dehydrogenase (LDH) was released and TBARS content increased. Ebselen (10, 25, 50 mu-mol cntdot L-1) reduced LDH efflux induced by O-2- and cntdot OH in a dose-dependent manner. As for the TBARS content, from 5 mu-mol cntdot L-1 to 50 mu-mol cntdot L-1, ebselen negated its increase, also dose-dependently. Furthermore, ebselen lowered TBARS content of cortical mitochondria treated with O-2- and cntdot OH in a dose-related manner. But ebselen showed no activity of scavenging O-2- and cntdot OH. This suggests that ebselen has direct anti-oxidant activity on neuron and its activity is not achieved by scavenging oxygen free radical.