Effects of 12/15-lipoxygenase antisense oligonucleotide on peroxisome proliferator-activated receptor γ translocation in primarily cultured cortical neurons after oxygen-glucose deprivation
Han, J.; Sun, L.; Liang, H.; Cheng, Y.
Zhonghua Yi Xue Za Zhi 94(15): 1179-1183
2014
ISSN/ISBN: 0376-2491 PMID: 24924720 Document Number: 672301
To explore the effects of 12/15-lipoxygenase antisense oligonucleotide (asON-12/15-LOX) on OGD (oxygen-glucose deprivation)-induced PPARγ (peroxisome proliferator-activated receptor γ) expression and nuclear translocation in primarily cultured cortical neurons. After a 48-h pre-treatment of 12/15-LOX antisense oligonucleotide (asON), primarily cultured cortical neurons underwent 3-hour OGD followed by a 24-hour reperfusion.Immunofluorescent staining and Western blot were used to evaluate the expressions of 12/15-LOX and PPARγ as well as the nuclear translocation of PPARγ. Compared with the control group, the expressions of 12/15-LOX and PPARγ whole protein were enhanced in OGD group (t = -3.72 and -6.79, P = 0.03 and 0.04). And an increase of PPARγ in nucleus (t = -4.67, P = 0.02) could be noted with a simultaneous reduction in cytosol (t = 3.40, P = 0.04) after OGD, indicating an induction of nuclear translocation by OGD. Compared with OGD group, a pre-treatment of asON-12/15-LOX dramatically attenuated OGD-induced increase in 12/15-LOX whole protein expression (t = 5.03, P = 0.02). Compared with OGD group, a pre-treatment of asON-12/15-LOX greatly reduced OGD-induced increase in PPARγ total protein expression (t = 2.83, P = 0.04) and nuclear translocation (t = 7.05, P = 0.01 for nuclear protein; t = -5.47, P = 0.01 for cytosol protein). It indicated a possible link between 12/15-LOX and PPARγ. 12/15-LOX antisense oligonucleotide suppresses the expression and nuclear translocation of PPARγ in primarily cultured cortical neurons after OGD.