Comparative study of the level of cardiomyocyte hypertrophy in patients suffering from ischemic heart disease with normal arterial pressure and in hypertension
Selivanova, G.V.; Vlasova, T.D.; Moiseeva, O.M.; Krutikov, A.N.; Gordeev, M.L.; Khirmanov, V.N.
Tsitologiia 39(10): 938-945
1997
ISSN/ISBN: 0041-3771 PMID: 9505341 Document Number: 474764
A study was performed on the human atrial myocytes, isolated by means of alkaline dissociation of bioptates, obtained in clinics during open heart surgery, from two groups of patients. The first group consisted of 8 men aged from 40 to 62 years, with ischemic heart disease and normal arterial blood pressure. The second group involved 10 men, aged from 43 to 67 years, with ischemic heart disease and arterial hypertension. The nuclear DNA and the total protein in the cytoplasm of isolated cardiomyocytes were revealed by means of the two consecutive tests: the Feulgen and Naphthol yellow S staining. DNA and protein contents were determined by two wave-length scanning cytophotometry. It is ascertained that the temperate arterial hypertension exerted no appreciable influence on the polyploidy level of atrial myocytes, which correlated with the age of patient; in both groups (coefficients 0.7 and 0.4 for the first and second groups, resp.). The myocyte areas correlate with their ploidy (coefficients--0.7 and 0.7, accordingly). The protein contents in myocytes also correlate with their ploidy (0.5 and 0.7). The average protein content in myocytes of patients in the second group is higher than in the first group, the difference is most distinct when calculating the protein quantity per 2c-genome (717 +/- 34 a. u. and 517 +/- 51 a. u., resp.). A comparison of cytochemical and morphometric indices with some clinical findings obtained by means of echocardiography allowed to reveal the presence of correlative connections among them. The protein contents in myocytes correlate with the left ventricle mass (coefficients 0.6 and 0.5 for the first and second groups, resp.). The correlation between the rate of transmitral blood flow and myocyte ploidy (0.8 and 0.9), and the correlation between the myocyte area and the rate of transmitral blood flow (0.8 and 0.8) look convincing.