Induction of elastase biosynthesis in Streptomyces sp.82
Vlakhov, S.; Dalev, P.; Kabadzhova, P.; G'oreva, V.
Antibiotiki i Khimioterapiia 41(4): 16-22
1996
ISSN/ISBN: 0235-2990 PMID: 8967805 Document Number: 463968
The effect of 6 nonspecific substrates such as tryptose, caseic acid, Hamerstene casein, tryptone, yeast extract and fish meal on the production of extracellular elastase was studied. Tryptone, yeast extract and fish meal were shown to have the inducing effect. The highest effect was observed with the use of fish meal. The effect of yeast extract was slightly lower. The optimal concentrations of the inductors were: 1 per cent for fish meal and 0.5 per cent for yeast extract. Two fractions were isolated from fish meal: protein concentrate with the molecular weight of more than 10,000 and amino acid concentrate with the molecular weight of 200-500. The induction of the biosynthesis of streptomycete elastase was observed only in the presence of the first fraction. The enzymatic hydrolysis of the protein fraction resulted in the formation of three new fractions: fraction A with the molecular weight of 3000, fraction B with the molecular weight of 1500 and fraction C with the molecular weight of 1000. Fraction A was the only one with the inducing effect.