Human granulocyte elastase analysis on a clinical analyser (Greiner G-400) and comparison with the manual IMAC-Elastase method
Tsaka, T.; Herkner, K.R.; Mühl, A.
European Journal of Clinical Chemistry and Clinical Biochemistry 30(2): 107-109
1992
ISSN/ISBN: 0939-4974 PMID: 1581410 Document Number: 399111
The performance of the human granulocyte elastase (EC 3.4.21.37) determination on the clinical analyser Greiner G-400 was evaluated. The detection limit of the assay is 4.68 micrograms/l. With pooled plasma samples, within-run variation (CV) ranged from 1.3 to 6.0%, between-run imprecision was 2.7 to 6.3%. Comparison with the homogenous immunoactivation manual assay using samples from 40 patients resulted in a very good correlation (r = 0.987). This suggests that the adapted instrumental method is able to replace the manual method for routine analyses.