Comparison of sperm preparation methods. Wash and concentration, swim-up, migration-gravity sedimentation, 80% Percoll and semen filtration column
Matsuoka, I.; Fujino, Y.; Ito, F.; Koh, B.; Kojima, T.; Ogita, S.
Journal of Reproductive Medicine 40(5): 342-346
1995
ISSN/ISBN: 0024-7758 PMID: 7608872 Document Number: 440480
The removal of all debris and background, as well as antifertility factors present in seminal fluid, may increase the fertilizing ability of spermatozoa. A number of semen manipulative techniques are currently available to remove the undesirable spermatozoa, debris and other factors and to increase sperm quality before performance of the various assisted reproductive technologies. We compared the impact of the wash and concentration method, swim-up method, 80% Percoll method, migration-gravity sedimentation method and SpermPrep method on postmanipulation sperm quality.