Comparison between colorimetric and column chromatographic methods for the determination of glycosylated hemoglobin
Vichayanrat, A.; Ploybutr, S.; Nitiyanant, W.; Vannasaeng, S.
Journal of the Medical Association of Thailand 67(5): 278-283
1984
ISSN/ISBN: 0125-2208 PMID: 6481271 Document Number: 238534
A study on glycosylated Hb (GHb) determined by colorimetry (2-thiobarbituric acid method) and column chromatography in 82 diabetics and normal subjects was reported. There was a good correlation between the GHb values measured by both methods (r = 0.879). An equation was derived from the curve and used to convert GHb by colorimetry in .mu.mol/l HMF (5-hydroxymethylfurfural) to total Hb percentage. In another group of 36 normal subject and 190 diabetics, GHb concentrations were determined by the colorimetric method and expressed as total Hb percentage. The mean GHb values of these 36 normal subjects, 20 untreated diabetics, 70 diabetics on insulin therapy and 100 diabetics on oral hypoglycemic agents were 6.64, 14.01, 13.59 and 11.70%. All diabetics had a significantly higher mean GHb values compared to normal subjects. The patients on oral hypoglycemic agents had a significantly lower GHb concentration when compared to untreated and insulin requiring diabetics. GHb determination by this colorimetric method expressed as total Hb percentage is accurate, convenient to standardize and inexpensive making it a suitable procedure for GHb measurement.