Separation of osteoclasts from bird medullary bone by sedimentation at unit gravity
Teti, A.; Pace, G.; Zallone, A.Z.
Bollettino della Societa Italiana di Biologia Sperimentale 57(1): 8-14
1981
ISSN/ISBN: 0037-8771 PMID: 7248107 Document Number: 176268
A procedure for the isolation of osteoclasts from the heterogeneous population of medullary bone and marrow cells is presented. Cell suspensions were prepared from medullary bone of laying hens by mechanical dispersion. Following an osmotic shock and 2 90 min unit gravity sedimentations, fractions highly enriched in osteoclasts were collected and subsequently cultivated in Eagle's minimal essential medium with fetal calf serum. Cytosine arabinoside was added to the cultures for 48 h to remove all the cells entering the mitotic cycle. The advantages and the possible applications of the method are discussed.