Formation of osteoclasts from blood monocytes during 1 alpha-OH Vit D-stimulated bone resorption in mice
Tinkler, S.M.; Linder, J.E.; Williams, D.M.; Johnson, N.W.
Journal of Anatomy 133(Part 3): 389-396
1981
ISSN/ISBN: 0021-8782 PMID: 7328045 Document Number: 180056
In order to investigate a monocyte origin for osteoclasts, tritiated thymidine labelled blood monocytes, harvested from the blood of donor mice, were injected intravenously into syngeneic recipient animals in which osteoclast formation was being stimulated by concomitant intraperitoneal injections of 1 alpha-hydroxycholecalciferol. Labelled osteoclasts were found in autoradiographs prepared from the femurs of recipient mice, demonstrating for the first time that, during hormonally stimulated osteoclast formation, blood monocytes form one source of osteoclasts.