Gel filtration and sedimentation behaviour of receptor-gonadotrophin complexes from rat ovary
Jääskeläinen, K.; Rajaniemi, H.; Reichert, L.E.
Acta Endocrinologica 97(2): 258-269
1981
ISSN/ISBN: 0001-5598 PMID: 6263030 Document Number: 172721
Membrane particles of luteinized rat ovaries and immature and mature granulosa cells of rat ovarian follicles were labeled with oFSH formed non-specific complexes with ovarian as well as with non-gonadal membrane components. The sedimentation coefficient of the non-specific complexes was 1.3 .+-. 0.5 S and the Stokes radius 49 .ANG. The macroscopic physical properties of the receptor-hCG-complex do not change during the maturation and subsequent luteinization of the granulosa cells. The methods used were not discriminating enough to reveal the existing structural differences between receptor-oFSH-complex and receptor-hCG-complex.