Effects of cisplatin and selenite on the level of thiols in pig blood platelets
Wachowicz, B.; Krajewski, T.; Olas, B.; Zbikowska, H.M.
Journal of Physiology and Pharmacology An Official Journal of the Polish Physiological Society 46(1): 97-103
1995
ISSN/ISBN: 0867-5910 PMID: 7599343 Document Number: 439169
The level of free sulfhydryl groups of glutathione and proteins in pig blood platelets after short lasting incubation (30 min.) of these cells with cisplatin (cis-diamminedichloroplatinum II) and sodium selenite was investigated in vitro. The concentration of the platelet thiols after sodium selenite or cisplatin treatment distinctly decreased (p < 0.001). Pretreatment of blood platelets with sodium selenite at the low concentration (0.1 microM) protected platelet glutathione against the reaction with cisplatin (p < 0.001). The present study shows that in blood platelets after the incubation with cisplatin the GS-platinum complex was formed. The results of our experiments demonstrated also that the extracellular GSH (1 mM) had the protective effect against cisplatin-induced peroxidation of platelet lipids.