Cloning in the lambda ZAPII/pBluescript system. I. Design of common cDNA libraries
Evtushenko, V.I.
Molekuliarnaia Biologiia 28(4): 778-789
1994
ISSN/ISBN: 0026-8984 PMID: 7990805 Document Number: 438130
Here in I summarize some cloning tips that have been revealed during construction of cDNA libraries from rat tissues and mouse stem cell lines in lambda ZAPII, and that improve the cloning efficiency. These improvements can be applied to many different vectors and cDNA cloning systems.
Document emailed within 1 workday
Related Documents
Williamson, C.L.; Slocum, R.D. 1994: Isolation of cDNA clones by complementation of E. coli mutants with infective pBluescript phagemid libraries Biotechniques 16(6): 986-988Lazaridis, K.; Dang, H.; Talal, N. 1993: Human kappa chain expression in a lambda phage vector: methods of isolating amplified cDNA affects cloning efficiency Human Antibodies and Hybridomas 4(3): 104-106
Jitsurong, S.; Chirathaworn, C.; Brown, N.F.; Beacham, I.R.; Tumwasorn, S. 2003: Searching for virulence Burkholderia pseudomallei genes by immunoscreening the lambda ZAPII expressed genomic library Southeast Asian Journal of Tropical Medicine and Public Health 34(4): 810-821
Hayzer, D.J.; Jaton, J.C. 1987: Nucleotide sequence of a cDNA clone encoding a rabbit immunoglobulin-lambda light chain: the V lambda region differs markedly from that of other species Journal of Immunology 138(7): 2316-2322
Li, H.; Fu, G.; Qin, Y.; Du, H.; Jiang, X.; Liu, M.; Kong, X. 2002: Cloning of AE1-c-end cDNA and construction of its expression plasmid for yeast two-hybrid system Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 19(2): 284-286; 290
Endege, W.O.; Steinmann, K.E.; Boardman, L.A.; Thibodeau, S.N.; Schlegel, R. 1999: Representative cDNA libraries and their utility in gene expression profiling Biotechniques 26(3): 542-8 550
Harline, M.C.; Kandala, J.C.; Sage, R.D.; Guntaka, R.V.; DeAngelo, A. 1992: Improved method for screening cDNA expression libraries for DNA-binding proteins Biotechniques 13(3): 388-391
Pippin, J.; Contractor, V.; Ambrus, J.L. 1994: An inexpensive method for the rapid identification of specific cDNAs from cDNA libraries Biotechniques 16(4): 676-8 680
López-Fernández, L.A.; del Mazo, J. 1993: Construction of subtractive cDNA libraries from limited amounts of mRNA and multiple cycles of subtraction Biotechniques 15(4): 654
Watanabe, K. 1990: CDNA cloning of PGF synthase Nihon Rinsho. Japanese Journal of Clinical Medicine 48(6): 1295-1302
Borovkov, A.I. 1986: New method for the synthesis and cloning of cDNA Genetika 22(1): 172-174
Takumi, T.; Lodish, H.F. 1994: Rapid cDNA cloning by PCR screening Biotechniques 17(3): 443-444
Herrmann, J.; Lee, P.; Saya, H.; Nakajima, M. 1990: Application of polymerase chain reaction for rapid subcloning of cDNA inserts from lambda gt11 clones Biotechniques 8(4): 376-381
Liao, Y.C.; Tokes, Z.; Lim, E.; Lackey, A.; Woo, C.H.; Button, J.D.; Clawson, G.A. 1987: Cloning of rat "prion-related protein" cDNA Laboratory Investigation; a Journal of Technical Methods and Pathology 57(4): 370-374
Födinger, M.; Ortner, S.; Plaimauer, B.; Wiedermann, G.; Scheiner, O.; Duchêne, M. 1992: cDNA cloning of Entamoeba histolytica histone H3 Archives of Medical Research 23(2): 19-21
Kwon, B.S.; Haq, A.K.; Kim, G.S.; Pomerantz, S.H.; Halaban, R. 1988: Cloning and characterization of a human tyrosinase cDNA Progress in Clinical and Biological Research 256: 273-282
Li, L.; Gong, H.; Wen, Y.; Chen, J.; Wang, R. 2002: The cloning and sequencing of H-2Kk gene cDNA of 615 mice Hua Xi Kou Qiang Yi Xue Za Zhi 20(5): 313-315
Darvas, F. 2000: Retrometabolism design concepts and realization for combinatorial libraries Die Pharmazie 55(3): 243
Meyer, L.; Heinrich, H.W.; Weber, S. 1987: CDNA cloning of foot-and-mouth disease virus Archiv für Experimentelle Veterinarmedizin 41(5): 665-670
Skalińska, B.A.; Pieniazek, N.; Stańczak, J.; Osowiecki, H. 1983: Cloning of Aspergillus nidulans ribosomal DNA in shotgun experiments using the EK2 vector lambda gtWEST.T5-622 Acta Microbiologica Polonica 32(3): 231-236