Cloning of AE1-c-end cDNA and construction of its expression plasmid for yeast two-hybrid system
Li, H.; Fu, G.; Qin, Y.; Du, H.; Jiang, X.; Liu, M.; Kong, X.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 19(2): 284-286; 290
2002
ISSN/ISBN: 1001-5515 PMID: 12224301 Document Number: 546451
In this study, about 350 bp cDNA fragment was amplified by PCR. After being sequenced, the AE1-c-end gene fragment was cloned into EcoR I-Pst I site of pGADT7 to form AD ends in the yeast two-hybrid system. The recombinant plasmid was transformed into yeast AH109, and the expression in the yeast was observed. The results demonstrate that AE1-c-end was obtained. pGADT7-AE1-c-end has no toxic effect on the yeast. It can serve as a target gene of yeast two-hybrid system.