The use of the two-hybrid cloning in yeast for functional characterization of protein kinase MAK-V
Korobko, I.V.; Korobko, E.V.; Chupikova, N.I.; Vanechkin, M.A.; Smirnova, E.V.; Kiselev, S.L.; Georgiev, G.P.
Molekuliarnaia Biologiia 36(3): 491-495
2002
ISSN/ISBN: 0026-8984 PMID: 12068635 Document Number: 548031
Identification of interaction partners opens a way to direct functional characterization of proteins. Several cDNAs coding for potential partners of protein kinase MAK-V/Hunk were isolated using two-hybrid cloning in yeast. Based on the partner properties, MAK-V/Hunk was assumed to play a role in tumorigenesis and tumor progression. With the previous results of two-hybrid cloning, MAK-V/Hunk was shown to participate in vesicular transport.