Androgen receptor isoforms expression and its novel cDNA cloning in human prostate
Xia, S.; Wang, Y.; Tang, X.
Zhonghua Yi Xue Za Zhi 81(24): 1500-1503
2001
ISSN/ISBN: 0376-2491 PMID: 16200775 Document Number: 527346
To investigate the androgen receptor isoforms expression and its novel eDNA cloning in prostate tissue. The tissue from a case of benign prostatic hyperplasia (BPH) was analyzed by high resolution isoelectric focusing (IEF) technique and molecular biological technique of cDNA cloning was used to determine its AR isoforms expression and genes of AR isoforms. From the prostatic specimen, three types of AR isoforms were detected with pI values of 6.5, 6.0 and 5.3 respectively. A novel AR subtype cDNA cloning, named as PHAR-1 and including 1 913 nucleotides was isolated from the same BPH tissue. The GenBank accession number for the nucleotide sequence of this AR cDNA was BankIT372205 AF324243. The comparison between PHAR-1 and AR nucleotide sequence registered in GenBank showed that 961 nucleotides in three part of nucleotide sequence were the same, while the other 952 nucleotides were different. These three AR isoforms may originate from the same gene. The results may be important for the study of BPH, or prostatic growth. It is also valuable in the field of pharmacotherapy for treatment of BPH and prostatic cancer.