Human kappa chain expression in a lambda phage vector: methods of isolating amplified cDNA affects cloning efficiency
Lazaridis, K.; Dang, H.; Talal, N.
Human Antibodies and Hybridomas 4(3): 104-106
1993
ISSN/ISBN: 0956-960X PMID: 8357956 Document Number: 406406
Three common methods of isolating amplified DNA were evaluated for their effects on cloning efficiency in a phage vector designed to express human kappa chain. The "glass milk" technique gave higher cloning efficiency and protein expression than phenol-chloroform extraction or microfiltration. This shows that the quality of amplified cDNA should be considered when studying the human antibody repertoire using this vector system.