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Construction of linear plasmid vectors for cloning in Escherichia coli cells

Vostrov, A.A.; Malinin, A.Iu.; Rybchin, V.N.; Sravchevskiĭ, A.N.

Genetika 28(7): 186-188

1992

ISSN/ISBN: 0016-6758
PMID: 1427053
Document Number: 395513
Prophage of the temperate coliphage N15 is a linear plasmid with covalently closed ends. The central part of plasmid N15 genome responsible for vegetative phage growth was replaced by DNA fragments containing genes for selective markers which have unique restriction sites. As a result a family of linear plasmid vectors was constructed. Their size is about 20 kb and their capacity is comparable with that of cosmid vectors.

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