SOS-induction in the presence of the plasmid pKM101 in the bacterial cells Escherichia coli K12
Tiganova, I.G.; Rusina, O.Iu.; Andreeva, I.V.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 2006(1): 11-14
2006
ISSN/ISBN: 0208-0613 PMID: 16512604 Document Number: 599721
Induction of transcription by the plasmid pKM101 (mutability mediating derivate of the plasmid R46) of the sfiA gene controlling cell division and of the fruA gene encoding the fructose specific enzyme I I of the phosphoenolpyruvate-phosphotransferase system in intact cultures of Escherichia coli was studied. The genes under study were fused to the bacteriophage Mu d1 (Ap lac). Activation of the sfiA gene, a typical member of the SOS-regulon, was demonstrated to depend on the key genes of the SOS-system-recA and lexA. In contrast, the fruA gene that is non-inducible by the UV-light, a classical SOS-inducing agent, is not activated by the presence of the plasmid pKM101 in the bacterial cells. The data obtained suggest that the presence of pKM101 plasmid in the Escherichia coli cells induces a SOS-signal as a consequence of the plasmid DNA replication or its conjugative transfer.