Cloning of L-2 DNA in Escherichia coli pOL4 plasmid
Honigman, A.; Kronman, C.; Nur, I.; Greenberg, N.; Rottem, S.
Israel Journal of Medical Sciences 20(9): 793-796
1984
ISSN/ISBN: 0021-2180 PMID: 6096305 Document Number: 238655
A physical map of L-2 DNA was constructed using restriction endonucleases. Based on this map the five HincII-generated L-2 DNA fragments (A-E) were cloned into the SmaI site of Escherichia coli vector plasmid pOL4, that was designed to analyze promoters and transcriptional terminators. The insertion of the HincII-generated L-2 DNA fragments into this plasmid clearly demonstrated that a fragment (fragment E) with a size of 1.1 kbp carried a sequence that initiated transcription in E. coli.