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Cloning and study of the function of recA-like gene of Yersinia pestis in Escherichia coli cells

Suchkov, I.I.; Mishan'kin, B.N.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 5: 34-39

1989

ISSN/ISBN: 0208-0613
PMID: 2664489
Document Number: 338408
A 2 kb fragment of Yersinia pestis genome cloned in Escherichia coli cells of the strain HB101 contains a gene able to complement the recA-dependent deficiency of E. coli cells in UV-resistance, resistance to alkylating agents, UV- and MNNG-induced mutability. Cellular capability for homologous recombination in crosses with HfrH donor, derepressed synthesis of bacteriocins (colicin E1 and pesticin 1) is also complemented by the fragment in E. coli recA-strains. The obtained data suggest the functional homology of the cloned recA-like gene product with the product of E. coli recA-gene.

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Vostrov, A.A.; Malinin, A.Iu.; Rybchin, V.N.; Sravchevskiĭ, A.N. 1992: Construction of linear plasmid vectors for cloning in Escherichia coli cells Genetika 28(7): 186-188
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