cDNA cloning of Entamoeba histolytica histone H3
Födinger, M.; Ortner, S.; Plaimauer, B.; Wiedermann, G.; Scheiner, O.; Duchêne, M.
Archives of Medical Research 23(2): 19-21
1992
ISSN/ISBN: 0188-4409 PMID: 1340291 Document Number: 401400
Entamoeba histolytica has an unusual nuclear structure. Although nucleosome-like particles have been observed, no information about histones is currently available. In this study, the authors describe a complementary DNA clone with significant homology to H3 histones which was isolated from a lambda Zap library of pathogenic E. histolytica. The complete cDNA encodes a 15 kDa polypeptide, which is shorter by one residue than the human homologue. The amino acid sequence has only 69% identity with human H3.3 histone. The results of this study indicate that this divergence may contribute to the unusual chromatin structure of E. histolytica.