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Improved method for screening cDNA expression libraries for DNA-binding proteins

Harline, M.C.; Kandala, J.C.; Sage, R.D.; Guntaka, R.V.; DeAngelo, A.

Biotechniques 13(3): 388-391

1992

ISSN/ISBN: 0736-6205
PMID: 1389172
Document Number: 402673
The ability to successfully screen a lambda gt11 cDNA expression library for specific gene products that can bind to selected sequences of DNA depends on radioactive double-stranded DNA probes with high specific activity. We demonstrate here that probes labeled by the PCR are superior to probes made by the Klenow reaction. The use of these PCR-generated probes have facilitated our efforts to isolate recombinant phage containing putative DNA-binding gene products that recognized a 246-base pair transcriptional enhancer region of Rous sarcoma virus long terminal repeat.

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Pippin, J.; Contractor, V.; Ambrus, J.L. 1994: An inexpensive method for the rapid identification of specific cDNAs from cDNA libraries Biotechniques 16(4): 676-8 680
Solov'eva, O.V.; Kakuev, D.L.; Gibanova, N.V.; Lipkin, V.M. 2000: Deletion mutants of the natriuretic peptide receptor type B: expression of cDNA, purification and characteristics of proteins Bioorganicheskaia Khimiia 26(6): 433-441
Guérif, F.; Anegon, I.; le Mauff, B.; Soulillou, J.P.; Pourcel, C. 1995: Screening of a pig cDNA expression library with human serum in order to identify new xenoantigens Transplantation Proceedings 27(4): 2491
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Zhu, X.-L.; Wu, J.-Z.; Wen, D.-S.; Guo, Q.-Y.; Wang, J. 2006: Screening and identification of metastasis-related gene expression in tongue carcinoma with cDNA microarray assay Hua Xi Kou Qiang Yi Xue Za Zhi 24(2): 166-169
Stanley, K.K.; Luzio, J.P. 1984: Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins EMBO Journal 3(6): 1429-1434
Matsuda, T.; Kabat, E.A. 1989: Variable region cDNA sequences and antigen binding specificity of mouse monoclonal antibodies to isomaltosyl oligosaccharides coupled to proteins. T-dependent analogues of alpha(1----6)dextran Journal of Immunology 142(3): 863-870
Evtushenko, V.I. 1994: Cloning in the lambda ZAPII/pBluescript system. I. Design of common cDNA libraries Molekuliarnaia Biologiia 28(4): 778-789
López-Fernández, L.A.; del Mazo, J. 1993: Construction of subtractive cDNA libraries from limited amounts of mRNA and multiple cycles of subtraction Biotechniques 15(4): 654
Williamson, C.L.; Slocum, R.D. 1994: Isolation of cDNA clones by complementation of E. coli mutants with infective pBluescript phagemid libraries Biotechniques 16(6): 986-988
Goyal, K.C.; Sharma, Y.K.; Chaudhary, V.K.; Misra, U.K. 1984: An improved method for isolation of retinol-binding protein from goat serum Indian Journal of Biochemistry and Biophysics 21(1): 59-61
Naoum, P.C.; Campos, M.C.; Parenti, M.F.; Szymanski, A.M. 1980: An improved electrophoretic method for a screening program for haemoglobinopathies Experientia 36(7): 875-876
Cheng, J.; Wang, L.; Li, K.; Lu, Y.-Y.; Wang, G.; Liu, Y.; Zhong, Y.-W.; Duan, H.-J.; Hong, Y.; Li, L.; Zhang, L.-X.; Chen, J.-M. 2003: Screening of augmenter of liver regeneration-binding proteins by yeast-two hybrid technique Hepatobiliary and Pancreatic Diseases International: Hbpd Int 2(1): 81-84
Wang, J.; Feng, L.; Yu, W.; Xu, J.; Yang, H.; Liu, X. 2013: Removal of high-abundance proteins in plasma of the obese by improved TCA/acetone precipitation method Wei Sheng Yan Jiu 42(5): 741-747
Nyman, T.; Pekonen, F. 1993: The expression of insulin-like growth factors and their binding proteins in normal human lymphocytes Acta Endocrinologica 128(2): 168-172
Pukhal'skiĭ, A.L.; Belkin, Z.P. 1982: Improved method of identifying cells bearing antigen-binding receptors for lymphocytes in the rosette formation reaction Biulleten' Eksperimental'noi Biologii i Meditsiny 94(12): 110-112
Hawley, S.B.; Green, M.A.; Miles, L.A. 2000: Discriminating between cell surface and intracellular plasminogen-binding proteins: heterogeneity in profibrinolytic plasminogen-binding proteins on monocytoid cells Thrombosis and Haemostasis 84(5): 882-890
Nagata, K.; Okano, Y.; Nozawa, Y. 1997: Differential expression of low Mr GTP-binding proteins in human megakaryoblastic leukemia cell line, MEG-01, and their possible involvement in the differentiation process Thrombosis and Haemostasis 77(2): 368-375
Hidaka, M.; Inoue, J.; Yoshida, M.; Seiki, M. 1988: Post-transcriptional regulator (rex) of HTLV-1 initiates expression of viral structural proteins but suppresses expression of regulatory proteins EMBO Journal 7(2): 519-523