Deletion mutants of the natriuretic peptide receptor type B: expression of cDNA, purification and characteristics of proteins
Solov'eva, O.V.; Kakuev, D.L.; Gibanova, N.V.; Lipkin, V.M.
Bioorganicheskaia Khimiia 26(6): 433-441
2000
ISSN/ISBN: 0132-3423 PMID: 10923191 Document Number: 518506
The C type natriuretic peptide (CNP) is a peptide hormone stimulating vasorelaxation and inhibiting cell proliferation. CNP activates the type B natriuretic peptide receptor (NPR-B), known as the guanylate cyclase B membrane enzyme, which results in the cGMP release. To study functional properties of NPR-B, its gene fragments were expressed in methylotrophic yeasts Pichia pastoris. Conditions were found providing for secretion of functionally active recombinant proteins NPR-Bs and NPR-B1 into the cultural medium in a yield of 25 mg/ml culture. Their specific activity was 0.97 and 0.93 mumol cGMP min-1 mg-1 protein, respectively. It was shown that NPR-B belongs to the family of Ser/Thr protein kinases and can be autophosphorylated at the serine residues.