An inexpensive method for the rapid identification of specific cDNAs from cDNA libraries
Pippin, J.; Contractor, V.; Ambrus, J.L.
Biotechniques 16(4): 676-8 680
1994
ISSN/ISBN: 0736-6205 PMID: 8024789 Document Number: 431246
The identification of specific cDNAs by screening filters impregnated with DNA from phage or bacterial colonies is a time-consuming and expensive undertaking. We have developed a rapid and inexpensive method for identifying specific cDNAs that involves hybridization of cDNAs to specific oligonucleotides bound to nylon filters. Using this method, we have identified specific cDNAs for a human B-cell growth factor from different cDNA libraries. The total processing time was 3 days, and 30% of the cDNAs selected by this method were correct.