Representative cDNA libraries and their utility in gene expression profiling
Endege, W.O.; Steinmann, K.E.; Boardman, L.A.; Thibodeau, S.N.; Schlegel, R.
Biotechniques 26(3): 542-8 550
1999
ISSN/ISBN: 0736-6205 PMID: 10090998 Document Number: 508805
An increasing interest in gene expression profiles in human diseases has led to the use of microdissected tumors and biopsies in gene discovery approaches. Since many of these clinical samples yield extremely small amounts of RNA, reproducible methods are needed to amplify this RNA while maintaining the original message profile. Using the SMART(R) cDNA Synthesis Method, we show that high-, medium- and low-abundance transcripts can be amplified in a representative fashion and that the resulting cDNA can also be used as a complex probe to confirm gene expression differences identified by other techniques.