Top Abstract Purchase Related
Home
>
Node
>
Subnode

Localization of human sterol carrier protein 2 gene and cDNA expression in COS-7 cell

Yamamoto, R.

Hokkaido Journal of Medical Science 67(6): 839-848

1992

ISSN/ISBN: 0367-6102
PMID: 1483685
Document Number: 398570
Sterol carrier protein 2 (SCP-2) is known to be an important polypeptide in intracellular sterol and lipid movement. Here are reported the cloning and expression of a cDNA encoding human SCP-2, and the assignment of SCP-2 gene to human chromosome 1. The 1,229 by cDNA contains an open reading frame and the encoded polypeptide consists of 143 amino acids. This polypeptide is very similar in amino acid composition to human, bovine and rat liver SCP-2. The deduced amino acid sequence of this cDNA has a 20-residue amino-terminal leader sequence with mature SCP-2. Northern blot analysis suggests that there are 1.8 kb and 3.2 kb mRNA species in human liver poly (A)+RNA. PCR analysis of human times rodent somatic cell hybrid panels indicates that the gene encoding SCP-2 is on human chromosome 1. Transient expression of the human liver SCP-2 cDNA in COS-7 cells shows a 15.3KDa polypeptide and increased amounts of a 13.2KDa polypeptide. Coexpression of the SCP-2 cDNA with cholesterol side-chain cleavage enzyme and adrenodoxin cDNAs resulted in a 2.5-fold progestin synthesis in comparison with that of the steroidogenic enzyme system alone. These findings suggest that SCP-2 plays a role in regulating steroidogenesis, among other possible functions.

Document emailed within 1 workday
Secure & encrypted payments

Sawa, H. 1990: Angiotensinogen: gene expression and protein localization in human tissues Hokkaido Journal of Medical Science 65(2): 189-199
Motwani, M.; White, R.A.; Guo, N.; Dowler, L.L.; Tauber, A.I.; Sastry, K.N. 1995: Mouse surfactant protein-D. cDNA cloning, characterization, and gene localization to chromosome 14 Journal of Immunology 155(12): 5671-5677
Park, J.-S.; Noh, D.-Y.; Kim, S.-H.; Kim, S.-H.; Kong, G.; Chang, C.-C.; Lee, Y.-S.; Trosko, J.E.; Kang, K.-S. 2004: Gene expression analysis in SV40-immortalized human breast luminal epithelial cells with stem cell characteristics using a cDNA microarray International Journal of Oncology 24(6): 1545-1558
Dempsey, M.E. 1985: Sterol carrier protein Methods in Enzymology 111: 293-303
Bond, V.C.; Huang, M.B.; Person, B.; Hairston, R.; Ye, X.Y.; Saltarelli, M. 1997: Effects of the human immunodeficiency virus type 1 Rev protein on reporter gene and host T-cell gene expression Cellular and Molecular Biology 43(7): 995-1005
Sakata, K-ichi.; Yamamoto, H.; Matsumoto, Y.; Someya, M.; Hareyama, M. 2007: cDNA analysis of gene expression associated with DNA-dependent protein kinase activity International Journal of Oncology 30(2): 413-420
Cui, N.-Q.; Zhang, S.-K.; Cui, Y.-F.; Li, D.-H.; Chen, C.; Wu, X.-Z. 2005: Overexpression of sterol carrier protein-2 mRNA in patients with cholesterol gallstones Hepatobiliary and Pancreatic Diseases International: Hbpd Int 4(1): 117-120
Hudson, P.; John, M.; Crawford, R.; Haralambidis, J.; Scanlon, D.; Gorman, J.; Tregear, G.; Shine, J.; Niall, H. 1984: Relaxin gene expression in human ovaries and the predicted structure of a human preprorelaxin by analysis of cDNA clones EMBO Journal 3(10): 2333-2339
Ouyang, Q-chang.; Hu, C-ping.; Shi, L-jie. 2003: Non small cell lung cancer gene expression profiles by cDNA microarrays Hunan Yi Ke Da Xue Xue Bao 28(1): 9-12
Miyagawa, N.; Miyagawa, S.; Ishii, H.; Hayashi, K.; Sakamoto, K.; Aoki, T. 1978: Studies on carrier protein in contact dermatitis: localization of soluble epidermal carrier proteins by macrophage migration test Acta Dermato-Venereologica 58(1): 23-27
Kaneda, M. 2004: Analysis of the mechanism involved in the neuroendocrine-related gene expression in a small cell lung carcinoma cell strain by correlation profiling of cDNA array data Hokkaido Journal of Medical Science 79(5): 547-556
Von Bahr-Lindström, H.; Höög, J.O.; Jörnvall, H. 1989: Human alcohol dehydrogenase: structural comparisons at gene, cDNA, and protein levels Progress in Clinical and Biological Research 290: 167-180
Celeste, A.J.; Rosen, V.; Buecker, J.L.; Kriz, R.; Wang, E.A.; Wozney, J.M. 1986: Isolation of the human gene for bone gla protein utilizing mouse and rat cDNA clones EMBO Journal 5(8): 1885-1890
Verwanger, T.; Sanovic, R.; Aberger, F.; Frischauf, A-Maria.; Krammer, B. 2002: Gene expression pattern following photodynamic treatment of the carcinoma cell line A-431 analysed by cDNA arrays International Journal of Oncology 21(6): 1353-1359
Zhang, J.; Dai, J.; Zhao, E.; Lin, Y.; Zeng, L.; Chen, J.; Zheng, H.; Wang, Y.; Li, X.; Ying, K.; Xie, Y.; Mao, Y. 2004: cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI Acta Biochimica Polonica 51(4): 1051-1058
Liang, X.H.; Volkmann, M.; Klein, R.; Herman, B.; Lockett, S.J. 1993: Co-localization of the tumor-suppressor protein p53 and human papillomavirus E6 protein in human cervical carcinoma cell lines Oncogene 8(10): 2645-2652
Shang-Dong, W.; Yuan-Cui, T.; Rong-Zhong, R.; Dong-Liang, C.; Yi-Liu, M.; Yuan-Wang, G.; He, Z.; Wang, S.; Shao-Liang, J.; Zhang, P. 2012: Decreased Expression of Retinoblastoma Protein-Interacting Zinc-Finger Gene 1 in Human Esophageal Squamous Cell Cancer by DNA Methylation Clinical Laboratory 58(1-2): 41-51
Rosenthal, A.; Ulrich, R.; Billwitz, H.; Frank, R.; Blöcker, H. 1987: Chemical-enzymatic synthesis, cloning and expression of a synthetic gene coding for an env protein fragment of the human T-cell leukemia virus type 1 Nucleic Acids Symposium Series 18: 233-236
Seimiya, H.; Sawabe, T.; Inazawa, J.; Tsuruo, T. 1995: Cloning, expression and chromosomal localization of a novel gene for protein tyrosine phosphatase (PTP-U2) induced by various differentiation-inducing agents Oncogene 10(9): 1731-1738
Birnbaum, M.; Spiess, E. 1988: Amplification, expression and localization of the c-myc gene in BSp73 rat tumor cell lines Anticancer Research 8(6): 1185-1191