Gene expression profiling in chemoresistant variants of three cell lines of different origin
Johnsson, A.; Vallon-Christensson, J.; Strand, C.; Litman, T.; Eriksen, J.
Anticancer Research 25(4): 2661-2668
2005
ISSN/ISBN: 0250-7005 PMID: 16080509 Document Number: 593683
Background: Drug resistance is a major problem in clinical cancer chemotherapy. Several mechanisms of resistance have been identified, but the underlying genomic changes are still poorly understood. Materials and Methods: Gene expression profiling, using cDNA microarray, was performed in eight cell lines (K562 leukemia, MCF-7 breast cancer and S1 colon cancer) with acquired resistance against five cytostatic drugs; daunorubicin (DNR), doxorubicin (DOX), vincristine (VCR), etoposide (VP) and mitoxantrone (MX). Results: The resistant cell lines clustered together based on their type of origin. Several genes encoding ABC transporters were highly up-regulated, most notably ABCB1 (MDR1) and ABCB4 in several cell lines and ABCG2 (MXR) specifically in MX-resistant cell lines. A pronounced down-regulation of several histones was noted in the MCF-7-derived resistant sublines. Altered expression was also seen in, e.g., GSTs, topoisomerases, caveolins, annexins and CD44. Conclusion: These results will constitute a platform for further studies on specific pathways and biological processes involved in chemotherapy resistance.