An improved method for isolation of retinol-binding protein from goat serum
Goyal, K.C.; Sharma, Y.K.; Chaudhary, V.K.; Misra, U.K.
Indian Journal of Biochemistry and Biophysics 21(1): 59-61
1984
ISSN/ISBN: 0301-1208 PMID: 6541636 Document Number: 226152
An improved method for the isolation of retinol-binding protein (RBP) from goat serum is reported. The method involves the use of DEAE-cellulose, CM-Sephadex and Sephadex G-100 column chromatography. The isolated RBP has a MW 20,420 and gave A280/A330 nm ratio of 1.01. The RBP was immunologically pure. The method is simple, less time consuming and reproducible.
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