Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)
Floccari, M.E.; Tiscornia, G.; Palmada, F.; Méndez, B.S.
Revista Argentina de Microbiologia 23(1): 26-29
1991
ISSN/ISBN: 0325-7541 PMID: 1815264 Document Number: 380334
Centrifugation through sucrose gradients was adapted to separate spore-forming cells of B. megaterium deficient in poly-beta-hydroxybutyrate synthesis from wild type cells. The conditions described allowed the detection of mutant clones screening a low percentage of the mutagenized population.
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