Methylobacterium extorquens strain P14, a new methylotrophic bacteria producing poly-beta-hydroxybutyrate (PHB)
Ostafin, M.; Haber, J.; Doronina, N.V.; Sokolov, A.P.; Trotsenko, Y.A.
Acta Microbiologica Polonica 48(1): 39-51
1999
ISSN/ISBN: 0137-1320 PMID: 10467695 Document Number: 506640
Strain P14 of facultative methylotrophic bacteria that synthetisizes poly-beta-hydroxybutyrate has been isolated. The cells are gram-negative motile rods with a polar flagellum. They do not form spores or capsules, but do have a caretenoid pigment. Predominant in the fatty acid composition of the cells is cis-vaccenic acid (cis 18 : 1 : omega7) - 72%. In the phospholipid composition phosphatidylcholine predominates (45%), along with phosphatidylenthanoloamine (27%) and phosphatidylglycerol (17%). The main ubiquinone is Q-10; other ubiquinones (Q-8, Q-9, Q-11) are present in minor quantities. The cells accomplish the icl- variant of serine pathway. The GC content of DNA (Tm) is 65 mole %. A high level DNA-DNA homology with representatives of the genus Methylobacterium was observed. The strain has been identified as Methylobacterium extorquens strain P14.