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NAD-dependent formate dehydrogenase from methylotrophic bacteria Pseudomonas sp. 101. I. Amino acid sequence

Popov, V.O.; Shumilin, I.A.; Ustinnikova, T.B.; Lamzin, V.S.; Egorov, T.A.

Bioorganicheskaia Khimiia 16(3): 324-335

1990

ISSN/ISBN: 0132-3423
PMID: 2357236
Document Number: 362548
The primary structure of NAD-dependent formate dehydrogenase from methylotrophic bacterium Pseudomonas sp. 101 is determined. The enzyme is composed of two identical subunits, each comprising 393 amino acid residues, and has a molecular weight of 43.1 kD. To elucidate the protein's amino acid sequence, four types of digestion were used: cyanogen bromide cleavage at methionine residues, endoproteinase Lys-C digestion at lysine residues, endoproteinase Glu-C cleavage at glutamic acid residues, and tryptic digestion. The peptides obtained were purified to homogeneity and characterized.

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