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The nucleotide sequence of the luxA and luxB genes of Xenorhabdus luminescens HM and a comparison of the amino acid sequences of luciferases from four species of bioluminescent bacteria

Johnston, T.C.; Rucker, E.B.; Cochrum, L.; Hruska, K.S.; Vandegrift, V.

Biochemical and Biophysical Research Communications 170(2): 407-415

1990

ISSN/ISBN: 0006-291X
PMID: 2383248
Document Number: 364187
The luxA and luxB genes of bioluminescent bacteria encode the .alpha. and .beta. subunits of luciferase, respectively. Sequences of the luxA and luxB genes of Xenorhabdus luminescens, the only terrestrial bioluminescent bacterium known, were determined and the amino acid sequence of luciferase deduced. The .alpha.-subunit was found to contain 360 amino acids and has a calculated molecular weight of 41,005 Da, while the .beta. subunit contains 327 amino acids and has a calculated molecular weight of 37,684 Da. Alignment of this luciferase with the luciferases of three marine bacteria showed 196 (or 55%) conserved residues in the .alpha. subunit and 114 (or 35%) conserved residues in the .beta. subunit. The highest degree of homology between any two species was between the luciferases of X. luminescens and Vibrio harveyi with 84% identity in the .alpha. subunits and 59% identity in the .beta. subunits.

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