Analysis of nucleotide sequences polymorphism of its regions of ribosomal genes of diploid Aegilops (L.) species
Goriunova, S.V.; Chikida, N.N.; Gori, M.; Kochieva, E.Z.
Molekuliarnaia Biologiia 39(2): 193-197
2005
ISSN/ISBN: 0026-8984 PMID: 15856941 Document Number: 584734
rDNA sequences of ITS region including full sequences of ITS1, 5.8S and partly sequenced ITS2 of 22 accessions of 5 diploid Aegilops species, were determined. The full alignment length was 524 bp. The analysis of ITS sequences shows a number of species-specific nucleotide changes. For the first time for diploid Aegilops species an interspecific polymorphism was shown. In some positions the polymorphism within accessions was determined. It can be differences either between or within individual plants. In general both inter- and intraspecific polymorphism of ITS sequences was very low. Nucleotide polymorphism was determined only in 25 sites, 12 of which were informative.