Enzymatic synthesis of beta-NAD+ selectively marked with tritium at adenine and its use for determining the activity of poly(ADP-ribose) polymerase

Shram, S.I.; Rybakova, I.G.; Lazurkina, T.I.; Sidorov, G.V.; Shilovskiĭ, G.A.; Khokhlov, A.N.; Miasoedov, N.F.; Shram, S.I; Rybakova, I.G; Lazurkina, T.I; Sidorov, G.V; Shilovskii, G.A; Khokhlov, A.N; Miasoedov, N.F

Home

>

Node

>

Subnode

Enzymatic synthesis of beta-NAD+ selectively marked with tritium at adenine and its use for determining the activity of poly (ADP-ribose) polymerase

Shram, S.I.; Rybakova, I.G.; Lazurkina, T.I.; Sidorov, G.V.; Shilovskiĭ, G.A.; Khokhlov, A.N.; Miasoedov, N.F.

Prikladnaia Biokhimiia i Mikrobiologiia 35(6): 638-646

1999

ISSN/ISBN: 0555-1099

PMID: 10623965

Document Number: 510279

A method of preparation of tritiated beta-HA [symbol: see text]+ from (adenine-2,8-(3)H)ATP and nicotinamide mononucleotide is suggested. This method is based on the baker's yeast (Saccharomyces cerevisiae) enzyme nicotinamide nucleotide adenylyltransferase. Experimental conditions for a nearly complete conversion of labeled ATP to NAD were determined. The tritiated NAD prepared by this method can be used in the quantitative assay of the chromatin enzyme poly(ADP-ribose) polymerase.