A new method for selective diagnostic staining of hooks of echinococci, cysticerci and tapeworms in histological sections
Stĕrba, J.; Milácek, P.; Vítovec, J.
Folia Parasitologica 36(4): 341-344
1989
ISSN/ISBN: 0015-5683 PMID: 2484916 Document Number: 332905
A new method for selective staining of hooks of echinococci, cysticerci and tapeworms in histological sections is described. It is based on pre-staining in haematoxylin, followed by staining overnight in dilute polychrome blue and differentiation with tartrazin in Cellosolve (ethylene glycol monoethyl ether or ethylene glycol monomethyl ether). The hooks stain blue, nuclei brown to brown-green, and cell plasma is yellow. The hooks stain very brightly, which enables the identification of parasite remnants in granulomata and scars. The intensity of staining depends on the grade of maturity and sclerotization of hooks and these are readily observed, even in lower magnifications. The staining was successfully used in a variety of parasites.