Pappenheim method of panoptic staining of histological sections
Tverdynin, M.S.; Bulanova, T.P.; Chernysheva, E.S.
Arkhiv Patologii 42(1): 80-81
1980
ISSN/ISBN: 0004-1955 PMID: 6155114 Document Number: 155764
A new method of azure-eosin staining of histological preparations during fixation with a 10% formalin solution, as well as of the cartilaginous and medullar tissues after decalcification in the 10% trilon B solution, pH 7,3 or in the de Castro liquid is described. This method has a number of advantages over the method os hematoxylin-eosin staining: it presents not only a clear idea about all cellular elements but also allows one to stain mast cells, fungi, microbes, Rickettsia, mucus, fibrin, necrobiotic changes in cells. Differentiation of hemopoietic cells and elements of reticuloendothelium, histiocytes is possible in the hemopoietic tissue. The method is accessible and simple and does not require special equipment.