Method of dissecting small sections while preserving the histological structural orientation of organs for electron microscopic studies
Gevondian, T.A.; Avtandilov, G.G.
Arkhiv Patologii 44(6): 66-68
1982
ISSN/ISBN: 0004-1955 PMID: 6751291 Document Number: 196110
A simple method of marking of the direction of the histostructures to be studied in a tissue block intended for electron microscopic examinations during dissection has been developed. The essence of the method is in dissecting pieces of an organ in the form of a parallelepiped of 0.7 X 0.7 X 1.1 mm in size so that the structures to be studied would have a known orientation in relation to the long axis of the parallelepiped. One general schematic map of muscle fibers direction has been made on the basis of layer-by-layer separation of fibers of myocardial bundles and comparison of their directions in heart ventricle walls of mature mice, rats, rabbits, dogs, and man. Using this map, it is possible to dissect a piece of the myocardium with the required orientation of muscle fibers and capillaries from any layer and part of each wall of the heart ventricles. A special knife having two pairs of parallelly located blades is proposed.