Method of detecting alkaline phosphatase and 5-nucleotidase in histological bone sections
Chernysheva, E.S.; Tverdynin, M.S.
Arkhiv Patologii 43(6): 75-77
1981
ISSN/ISBN: 0004-1955 PMID: 6268027 Document Number: 173687
Modification of the methods of Gomory, Pearce, and Reis for the detection of alkaline phosphatase and 5-nucleotidase in the bone tissue is described. The authors used fixation of the bone tissue in acetone at a temperature of 0 to 4 degrees C for 6 to 12 hours, decalcification in Grip's fluid followed by cutting in a freezing microtome of incompletely decalcified bone material and its decalcification in sections. As a result, localization of the enzyme is detected by the distribution of black sediments of cobalt sulfide.