A modified staining method for connective tissue in semi-thin histological sections of peripheral and cranial nerves
Menovsky, T.; van den Bergh Weerman, M.
Acta Chirurgiae Plasticae 41(4): 120-122
1999
ISSN/ISBN: 0001-5423 PMID: 10743716 Document Number: 506164
A simple staining method for collagen in semi-thin sections of nerves is described. This technique consists of applying basic fuchsin (0.05%) solution for 2 min. to +/- 1.25 microns plastic resin-embedded sections after routine toluidine blue staining. This staining method clearly demonstrates the amount and orientation of collagenous connective tissue in the nerve, both in transverse and longitudinal sections.