Immunolabelling of freeze-fractured tissue: thin sections of rat aorta after labelling of elastic laminae
Ruggiero, F.; Lethias, C.; Garrone, R.
European Journal of Cell Biology 44(1): 90-92
1987
ISSN/ISBN: 0171-9335 PMID: 3622536 Document Number: 297362
Rat aortae were fixed, reduced into fragments, fixed again according to the fracture-label method from Pinto da Silva et al. (1981) and labelled with anti-elastin antibodies. We thus demonstrate that this method, extensively used with lectins, is also usable with antibodies to investigate components of the extracellular matrix.