Serologic studies with the virus of vesicular stomatitis. II. Typing of vesicular stomatitis convalescent serum by direct complement fixation
Jenney, E.W.; Mott, L.O.
American Journal of Veterinary Research 24: 874-879
1963
ISSN/ISBN: 0002-9645 PMID: 13957600 Document Number: 3171
A serologic technique was developed for testing convalescent serums to differentiate between New Jersey and Indiana types of vesicular stomatitis (VS) infection. The test used live-virus antigen produced in embryonating chicken eggs. According to the degree of complement fixation (CF), convalescent serum could be classified into (1) type specific, (2) type suspect, (3) specific for vesicular stomatitis but nontypeable, (4) nonspecific, and (5) negative. The infecting type of VS virus was indicated by the following type specific and suspect reactions considered together: 70% of the bovine and 50% of the equine serums at 2 weeks postinoculation (PI), 97% of the bovine and 90% of the equine serums at 1 month PI, and 85% of the bovine and 100% of the equine serums at 2 months PI. Of 52 uninoculated bovine controls, 96.0% were negative, and 3.8% were either classified as suspect or nonspecific. Of 45 uninoculated equine controls, 100% were negative.
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