Vesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts
Sláviková, M.; Kocáková, P.; Slovák, M.; Vancová, I.; Hajnická, V.; Gasperík, J.; Fuchsberger, N.; Nuttall, P.A.
Acta Virologica 46(2): 117-120
2002
ISSN/ISBN: 0001-723X PMID: 12387504 Document Number: 551455
A salivary gland extract (SGE) prepared from 5-day-fed Dermacentor reticulatus female ticks was fractionated by fast protein liquid chromatography (FPLC). The effect of three FPLC fractions selected on the basis of anti-interleukin 8 (anti-IL-8) activity on vesicular stomatitis virus (VSV) nucleocapsid (N) protein formation in mouse L-cells was determined. Infected 14C-labelled cells treated with the FPLC fractions were analysed by two-dimensional (2D) electrophoresis. The yields of VSV N protein were evaluated by Imagemaster software analysis. Most noticeable was an increase in the N protein production after treatment with the fraction 39 corresponding to the major peak of the anti-IL-8 activity. The nature of the substance in SGE that was responsible for this effect remains unclear.