UV spectrophotometric determination of hydralazine hydrochloride in tablets: collaborative study
Mopper, B.
Journal - Association of Official Analytical Chemists 71(6): 1121-1122
1988
ISSN/ISBN: 0004-5756 PMID: 3240965 Document Number: 310554
A UV spectrophotometric method for the determination of hydralazine hydrochloride in tablets was collaboratively studied by laboratories. The method is based on conversion of hydralazine to a tetraxlo [5,1-.alpha.]phthalazine derivative which shows an absorption maximum at about 274 nm. Each collaborator received blind duplicate samples of 2 commercial powdered composites from 10 and 100 mg tablets, and 1 synthetic tablet formulation. Each collaborator also received a set of 10 tables for determination of content uniformity. The pooled mean recovery of hydralazine hydrochloride from the synthetic formulation was 101.2 .+-. 0.94%. The mean assay values for 10 and 100 mg tablets were 95.6 .+-. 0.98 and 101. .+-. 0.73% of the declared amounts, respectively, with corresponding CV values of 1.02 and 0.73%. The pooled mean for individual tablet assay was 99.8 .+-. 3.26% of the declared value, with a CV of 3.29%. The method has been adopted official first action.