High performance liquid chromatographic determination of methapyrilene hydrochloride in feed and sleep aid tablets
Shaikh, B.; Hallmark, M.R.
Journal - Association of Official Analytical Chemists 64(4): 889-892
1981
ISSN/ISBN: 0004-5756 PMID: 7275906 Document Number: 178986
Methapyrilene hydrochloride (MP·HCl) was extracted from feed with methanol and determined by reverse phase partition chromatography in less than 15 min, using isocratic elution with acetonitrile-1.1% ammonium carbonate (1 + 1) as the mobile phase. This procedure was tested on feed treated with MP·HCl at levels of 125,500, and 2000 ppm. Recoveries were 104,95, and 96% with coefficients of variation of 2.4,1.6, and 0.6%, respectively. MP·HCl in feed was stable for 14 days. This method was also successfully used to determine MP·HCl in 3 sleep aid tablets.