High performance liquid chromatographic analysis of supplemental vitamin E in feed
Shaikh, B.; Huang, H.S.; Zielinski, W.L.
Journal - Association of Official Analytical Chemists 60(1): 137-139
1977
ISSN/ISBN: 0004-5756 PMID: 833088 Document Number: 112589
Supplemental vitamin E is extracted from feed in 1 step with methanol and analyzed by reverse phase partition chromatography in less than 10 min, using isocratic elution with either methanol or water-methanol as the mobile phase. Detection response (as measured by peak area) was linear between 0.5 and 3.0 .mu.g, and the coefficients of variation for retention time and peak height on replicate analyses of the standard sample were 0.8 and 2.3%, respectively.